Characterization of bacterial community in indoor environment

We report the presence and characterization of bacterial colonies in indoor environments by molecular microbiology techniques. The bacterial DNA was used as template in PCR experiments in order to amplify both specific 16S rRNA locus and the 16S-23S intergenic transcribed spacer (ITS). The sequence of amplified fragments were determined and analysed to elicit the prevalent bacterial species in the microbial community. Comparison between fragments sequences and bacterial genomic DNA deposited in Genebank allowed us to create phylogenetic dendrograms. In this work we also focalised our attention on characterization of bacterial population present in library bioaerosol and onto books housed, focusing on species involved in degradation of cellulose. Moreover, SEM observation, cellulase activity assay analyses were consistent with molecular results.