Solution structure of the C-terminal domain of multiprotein bridging factor 1 (MBF1) of Trichaderma reesei

Trichoderma reesei is a filamentous fungus which has important biotechnological applications.7 TrMBF1 was identified during the annotation of the EST cDNA library of T. reesei.8 To understand how MBF1 binds to TBP and to other transcription factors enhancing promoter transcription, it is important to have structure information about MBF1 itself, therefore we initiated the study of the three-dimensional structure of TrMBF1 by NMR. As the full-length protein is unstable in solution, we used a shorter construction corresponding to the C-terminal domain (residues 56-155) (Ctd-TrMBF1).