Beta1-integrin and TRPV4 are involved in osteoblast adhesion to different titanium surface topographies

This paper aimed to assess how four different and representative dental implant surfaces (machined MAC, grit blasted GB, acid etched AE and grit blasted acid etched GBAE) could affect cell adhesion possibly modulating protein adsorption and a paramount cell receptor such as ?5ß1 integrin. Based on non-contact 3D profilometry, GBAE was rougher than the other surfaces, while GB and AE reached similar intermediate S values and MAC resulted the smoothest one. According to X-ray Photoelectron Spectroscopy and Raman spectroscopy, all the surfaces showed amorphous titania with similar amounts of carbon contaminants. The lesser protein adsorption and delayed cell adhesion of GB, which did not hinder cell proliferation, were correlated to the altered dispersive to polar SE ratio, that was at least double-fold for GB (2.6) compared to MAC (0.8), SL (1.3) and AE (1.2). The biological response in vitro relied on ?1 integrin activation that cooperated with the putative mechano-protein transient receptor potential vanilloid 4 (TRPV4) in determining the adhesion of the osteoblasts. In fact, it could be rescued by over activating ?1 integrin. On the other hand, silencing TRPV4 strongly inhibited cell adhesion on selected substrates, proving the role of this protein in mediating osteoblasts adhesion on titanium substrates.