Separation of amino acid enantiomers by adding a chiral complex to the eluent

Amino acid enantiomers are separated by reverse-phase chromatography with an optically active polyamine/Cu (II) complex in the mobile phase. The polyamine was obtained by reaction of ( - ) -trans-1,2-diaminocyclohexane with ethylene glycol diglycidyl ether; the complex with copper (II) ions was used in the eluent. Separation factors are reported for ten amino acids and four barbiturates