Cyclodextrin Nanomagnetes Decorated With Peptides: From Abeta-Amyloid Detection To Theranostic

Magnetic nanoparticles (MNPs) are widely utilized in magnetic-field-assisted bio-separation, bio- interaction, imaging and drug delivery, due to their large surface area and easy manipulation by an external magnetic force. MNPs, stabilized with different capping agents and incorporating drugs, were designed as nanotheranostic. In this context, cyclodextrin-based MNPs offer a multipurpose chance in detection, therapy and imaging, since cyclodextrins (CDs) can strongly entrap in its cavity hydrophobic guests to optimize molecular recognition of biomarkers or for sustained therapeutic action in the site where they are directed with the help of the magnetic field and / or serving as contrast agents in magnetic resonance imaging (MRI) . Amphiphilic cyclodextrin (aCD) are efficaciously engaged to entrap and control deliver of drugs and diagnostic probes [1]. Here we propose MNP covered with aCD nanomagnets (aCD is heptakis (2-oligo(ethyleneoxide)-6-hexadecylthio-)-?- CD (SC16OH)) (MNP@SC16OH) and tailored with Adamantanil-(PEG)4-KLVFF (Ada-Pep) for recognition and detection of Amyloid-?(1-42) protein (A?(1-42)). MNP@SC16OH/Ada-Pep were prepared by hydration of an organic film of Ada-Pep with a dispersion of pre-made-up MNP@SC16OH. Hence, modified MNPs were isolated by magnetic-filed separation, centrifugation and fully characterized [2]. Furthermore, we investigated the ability of system to fish out A?(1-42) from samples prepared in aqueous solutions and analysed by MALDI-TOF MS. Our results indicate that in our experimental conditions the nanomagnets can perform an unbiased A?(1-42)-targeted fishing. Moreover the effect of nanomagnetes in delivery of anticancer drugs as sorafenib in hepatocellular carcinoma (HCC) cells as well as in in vitro enhancement of MRI relaxivity was investigated [3]. Altogether, results indicate the potential utilize of these nanomagnets based on cyclodextrins as therapeutic and imaging agents.