DETERMINATION OF SUGARS AND SUGAR ALCOHOLS IN DIETETIC SWEETENERS AND FOOD BY HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY (HPAEC) COUPLED WITH PULSED AMPEROMETRIC DETECTION (PAD)

In this study the effects of mobile phase composition on the chromatographic retention of neutral carbohydrates and sugar alcohols in dietetic sweeteners, as well as in processed food, have been examined by high-performance anion-exchange chromatography (HPAEC) using two different anion-exchange columns, marketed by Dionex as CarboPac PA-100 and CarboPac MA-1. Results on separation of saccharose substitutes in confectionary products by HPAEC-PAD have been discussed.