Self-assembling Releasable Thiocolchicine-Diphenylbutenylaniline Conjugates

The design and the synthesis of new self-assembling conjugates is reported. The target compounds are characterized by the presence of a self-immolative linker that secures a controlled release induced by lipase cleavage. 4-(1,2-Diphenylbut-1-en-1-yl)aniline is used as a self-assembling inducer and amino-thiocolchicine as prototype of drug. The release of thiocolchicine derivative has been demonstrated in vitro in the presence of porcine pancreatic lipase (PPL) and celite-supported lipase (PS). The formation of nanoparticles is confirmed by DLS, atomic force microscopy (AFM) and fluorescence microscopy. The antiproliferative activity has been proved on two human cancer cell lines.