Gold microtubes assembling architecture for an impedimetric glucose biosensing system.

A glucose impedimetric biosensor was assembled using a suggested gold microtubes (AuTs) archi-tecture. A platinum (Pt) electrode (diameter 3 mm) was coated by gold microtubes, synthesized viaelectroless deposition within the pores of polycarbonate particle track-etched membranes (PTM). Thisplatform was successfully used to deposit polypyrrole overoxidized film (OpPy) and to verify the pos-sibility of developing a biosensor using OpPy, the characteristics of the H2O2charge transfer reactionwere studied before the enzyme immobilization. This composite material could be suitable in devices asbiosensors based on oxidase enzymes, just because hydrogen peroxide is a side-product of the catalysisand could be directly related to the concentration of the analyte. Finally, a biosensor consisting in a Ptelectrode modified with AuTs, OpPy and glucose oxidase was assembled to determine the glucose.The most important result of this biosensor was the wide linear range of concentration, ranging from1.0 to 100 mM (18-1800 mg dl-1), covering the hypo- and hyperglycemia range, useful in diabetes, withlimit of detection (LOD) of 0.1 mM (1.8 mg dl-1) and limit of quantification (LOQ) 1.0 mM (18 mg dl-1).