Biochemical Comparison Between Clostridium Hystoliticum Collagenases G and H obtained by DNA Recombinant and Extractive Procedures

One of the main success practices in cell therapy is the transplantation of allogeneic pancreatic islets cells in patients with type 1 diabetes. However, at the moment less than 50 % of procedures are successfully completed; this is because the procedure of pancreatic islet isolation/purification, are not well standardized. This low percent of success can be ascribed to several factors, including: - the variability in donor characteristics, - pancreas recovery and preservation, - not the last, an unpredictable enzymatic blend efficiency.