A flexible modular bio-instrument for amperometric detection of bioenergetic material.

A flexible modular biosensor for amperometric detection suitable for bioenergetic material has been designed, fabricated and tested. The system is made up by two flow cells in series provided of screen printed electrodes to measure the output generated current and of a flow system for electrolytic solution. The bio-instrument could be used for biosensors using several biological materials (e.g. DNA, RNA and proteins). In this work RCcomplexes are used: photosynthetic electron transfer is activated by two LEDs (430 and 630nm) in order to enabling more excitations for different biological materials. RC-complexes with different sensitivity and selectivity to oxidants have been extracted from cyanobacteria (Synecococcus elongatus), algae (Chlamydomonas reinhardtii) and higher plants (Spinacea oleracea and Medicago sativa). Targeted applications belong to the agrofood, pharmaceutical and biomedical fields; in particular in this paper antioxidant power analysis has been explored.