Occurrence, origin and fate of pyroglutamyl-gamma3-casein in cheese

During plasmin-catalysed proteolysis of b-casein (b-CN) in cheese, pyroglutamyl-g3-casein (pg3-CN), originates from the cyclisation of the N-terminal Glu of g3-CN (b-CN f108e209). This peptide progressively increased in content during ripening of Grana Padano and Parmigiano-Reggiano Protected Designation of Origin cheeses. A preliminary survey revealed pg3-CN in both hard and extra-hard cheese varieties; in semi-hard cheeses it was present only in mature samples. To understand the mechanisms of pg3-CN formation/degradation, in vitro trials (real cheese, model cheeses and buffered g3-CN solutions) were performed. Both the effect of cheese pH and the capacity of certain microorganisms to cyclise the N-terminal Glu residue of g3-CN were studied. The results suggested that pg3-CN formed spontaneously during cheese ripening, mainly at the acid pH characteristic of hard and extra-hard cheeses; the role of cyclase enzymes was negligible. Differences in pg3-CN degradation were observed due to the proteolytic activity of bacteria involved in the cheese ripening.