Resonance as a Tool to Transfer Information to Living Systems: The Effect of 7 Hz Calcium Ion Energy Resonance on Human Epithelial Cells (HaCaT) Differentiation

The specific aim of the present work concerns the effectiveness of low frequency electromagnetic fields treatment to modify biochemical properties of human keratinocytes (HaCaT). Cells exposed to a 7 Hz electromagnetic field and 9.2 mu T static magnetic field (B-o) and 9 for one hour (twice daily), showed by Scanning Microscopy modification in shape and morphology; these modifications were also associated to different actin distribution revealed by phalloidin fluorescence analysis. Indirect immunofluorescence with fluorescent antibodies against involucrin and beta catenin, both differentiation and adhesion markers, revealed an increase in involucrin and beta catenin expression, supporting that exposure to electromagnetic field carries keratinocytes to an upper differentiation level.