Data di Pubblicazione:
2015
Abstract:
Despite
the
continuous
advancements
in
bio-molecular
detection
methods
and
flu
-
idic
integration
approaches,
the
realization
of
portable
and
high
performance
de
-
vices
for
diagnostic
applications
still
presents
major
difficulties,
mostly
due
to
the
need
of
combining
adequate
sensitivity
with
low
cost
of
production,
operational
simplicity
and
rapidity.
In
this
context,
we
have
previously
proposed
a
compact
device
composed
of
a
smartphone
and
a
custom-designed
cradle,
containing
only
a
disposable
sensing
cartridge,
a
tiny
magnetic
stirrer
and
a
few
passive
optical
components.
The
detection
principle
is
named
Reflective
Phantom
Interface
and
is
based
on
measuring
the
intensity
of
light
reflected
by
the
surface
of
an
amorphous
fluoropolymer
substrate
having
a
refractive
index
very
similar
to
that
of
water.
The
reflectivity
of
dozens
of
spots
is
monitored
in
real
time
by
the
smartphone's
camera
using
the
embedded
flash
LED
as
the
illumination
source.
We
tested
the
sensitivity
and
the
repeatability
of
the
combined
device
analyzing
multiple
spots
of
antibodies
targeting
an
antigen
commonly
used
as
marker
for
diagnoses
of
HIV.
Target
concen
-
trations
as
low
as
a
few
ng/ml
can
be
rapidly
and
robustly
determined
by
comparing
the
rate
of
increase
of
the
signal
after
the
addition
of
the
sample
with
that
measured
after
the
subsequent
addition
of
a
standard
solution
with
known
concentration
Tipologia CRIS:
01.01 Articolo in rivista
Keywords:
x
Elenco autori:
Sola, Laura; Damin, Francesco; Chiari, Marcella
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