Micro-raman spectroscopy for monitoring changes in periodontal ligaments and gingival crevicular fluid

Micro-Raman Spectroscopy is an efficient method for analyzing biological specimens due to its sensitivity to subtle chemical and structural changes. The aim of thisstudy was to use micro-Raman spectroscopy to analyze chemical and structural changes inperiodontal ligament after orthodontic force application and in gingival crevicular fluid inpresence of periodontal disease. The biopsy of periodontal ligament samples of premolarsextracted for orthodontic reasons and the gingival crevicular fluid samples collected byusing absorbent paper cones; were analyzed by micro-Raman spectroscopy. Changesof the secondary protein structure related to different times of orthodontic force applicationwere reported; whereas an increase of carotene was revealed in patients affected byperiodontal inflammation.