HPLC determination of tobramycin in a simulated body fluid

To evaluate the release kinetics, in a simulated body fluid, of tobramycin (TOB) from carbonated apatite (CHA) in the form Of granulate, a sensitive and reliable liquid chromatographic method was developed. Chromatographic separation was performed on a Metrosep Carb 1 anionic column by using 0.1 M NaOH as mobile phase at a flow rate of 1.0 ml/min. TOB was quantified by pulsed amperometric detection using a 3-potential waveform on Au electrode. A linear relationship for TOB was found in the range 0.17-2.50 mg mg/l with a LOD of 0.05 mg/l. The method exhibited good retention time and peak area reproducibility.