RECOMBINANT ALPHA-2A INTERFERON AND POLYCYTHEMIA-VERA - CLINICAL-RESULTS AND BIOLOGICAL EVALUATION BY MEANS OF FOURIER-TRANSFORM INFRARED MICROSPECTROSCOPY

Polycythemia vera (PV) is a chronic myeloproliferative disease. The use of recombinant alpha 2a Interferon (IFN) therapy in this disease is a novel approach. We applied Fourier-transform infrared microspectroscopy (FT-IR-M) to investigate the behavior and therapeutic responsiveness of PV patients treated with IFN. A spectroscopic parameter (A(1)/A(2)) was used, corresponding to the ratio of the integrated areas of the bands at 1080 cm(-1) and at 1540 cm(-1) due to nucleic acids and proteic components, respectively, calculated on the spectra of single megakaryocytes (MKs), In previous studies, we have pointed out that MKs in PV have a surprisingly strong myeloproliferative impulse when compared to MKs from other chronic myeloproliferative diseases. Nine patients out of the 11 studied exhibited a satisfactory responsiveness to the IFN treatment. Ten patients were evaluated by the A(1)/A(2) parameter. In 8 of these, a good agreement was seen between this parameter and the laboratory data commonly used for the assessment of this disease. The infrared parameter, which we propose, proves to be an original, reliable method for the evaluation of recombinant alpha 2a IFN responsiveness in this disease.