Low Cost Synthesis of Smart Biocompatible Graphene Oxide Reduced Species by means of GFP

The aim of this work is focused on the engineering of biocompatible complex systems composed by an inorganic and bio part. Graphene oxide (GO) and/or graphite oxide (GtO) were taken into account as potential substrate to linkage the protein such as Anemonia sulcata recombinant Green Fluorescent Protein (rAsGFP). The complex system is obtained through a reducing process between GO/GtO and rAsGFP archiving an environmentally friendly biosynthesis. Spectroscopic measurements supports the formation of reduced species. In particular, photoluminescence shows a change in the activity of protein when a bond is formed, highlighted by a loss of the maximum emission signal of rAsGFP and a red-shift of the maximun absorption peak of GO/GtO species. Moreover, the hemolysis assay reveals a lower values in the presence of less oxided graphene species providing evidence for a biocompatible material. This singular aspect can be approached as a promising method for circulating pharmaceutical preparations via intravenous administration in the field of drug delivery.