Resting Potentials recorded in the whole-cell configuration from epithelial cells of Hydra vulgaris

Gigaseals were obtained from enzymatically dissociated epithelial cells of Hydra vulgaris, and resting potentials were recorded in the whole-cell configuration. These results were obtained in spite of hard difficulties of applying patch-clamp techniques in the whole-cell configuration to Hydra cells. These difficulties are principally inherent to the fact that Hydra lives in a low ionic strength medium. It will be now possible to apply patch-clamp techniques to the study of Hydra extraocular photoreception.