Multifluorescence labeling and colocalization analyses.

Analyses with double-fluorescent labeling, such as three-dimensional reconstructions and/or quantification, require a sensitive detection system capable of resolving multiple fluorescent signals in a very accurate way. A general method for the imaging technique takes advantage of the particular properties of confocal laser scanning microscope (CLSM), which performs an optical sectioning of the sample by rejection of the out-of-focus light via a confocal pinhole.