Selective detection of 13C-containing metabolites by 1H double-resonance BLISS sequence

Recently there has been a great deal of interest in double-resonance methods capable to enhance the detection sensitivity of less sensitive nuclei such as "C in liquid system, in order to study brain metabolism noninvasively. The main disadvantage of the existing 'H-observed/"C-editing double resonance techniques, based on intermolecular short-range J-coupling interaction, is the nontotal proton solvent elimination. The indirect "C-editing and spatial localisation in 10 mM solution of enriched glucose in water, was obtained in our laboratory by means of the BLISS (bilinear and linear interaction spin-selection and solvent suppression) sequence with total solvent elimination. The sensitivity gain and the water suppression are here theoretically calculated and experimentally verified.