NIRS discrimination of broiler rabbits fed with increasing levels of false flax (Camelina sativa L.) seeds in relationships to the fatty acid profiles.

Three groups of ten young rabbits each received an enriched diet with false flax (Camelina sativa L.) seeds at 0% (C), 10% (M), and 15% (H), respectively. At the end of the experimental period, which lasted 50 days, all the rabbits were slaughtered. The longissimus dorsi (LD) muscle and perirenal fat samples were collected at 24 h post mortem from each carcass and analysed with a GC method for the fatty acid (FA) profiles and their indexes. Spectroscopy was conducted using a Model LSP LabSpec-Pro portable UV-Vis.NIR spectrophotometer (350-2500nm). The perirenal fat was directly examined. The samples derived from thawed LD muscles (2 cm ø x 2 cm long.) were previously fixed in 95% ethanol, stored for 3 days and finally scanned after 2 and 24 hours air exposition of the tissues. Discrimination of individuals between couple of groups, fitted 1 or 2 dummy values, was performed by a Modified Partial Least Square Discriminant Analysis by the WinISI II software (Infrasoft International, Port Matilda, PA, USA) allowing one passage for the removal of the outliers. The cross-validated 1-VR (Variance Ratio) coefficient was retained for comparative purposes..................