High-field NMR study of truffles (Tuber aestivum vittadini)

A high-field NMR technique was used to analyze aqueous and organic extracts of truffles (Tuber aestivum vittadini) to characterize their chemical composition. Water-soluble metabolites belonging to different classes such as sugars, polyols, amino acids, and organic acids were almost completely assigned by means of one- and two-dimensional experiments (H-1-H-1 COSY, TOCSY, H-1-C-13 HSQC, H-1-C-13 HMBC, and H-1-P-31 HMBC). The H-1 spectral assignment of the cell membrane components such as lipids, sterols, and fatty acids extracted in organic solvents was also performed.