Low molecular weight glutenin subunits in Triticum turgidum ssp. turanicum, polonicum and carthlicum.

The low-molecular weight glutenin subunits (LMW-GS) affect viscoelastic properties of dough. They have been subdivided, according to their biochemical properties, into B-, C- and D­types: the B types are typical LMW-GS, the C- and D-types correspond to modified monomeric proteins, similar to the so called gliadins. The LMW-GSs, mainly encoded by a multigene family present at the Glu-3 loci located on the short arms of the group 1 chromosomes closely link.ed to the Gli-I loci, have not been completely isolated and characterized in tetraploid wheat. In arder to study the polymorphism of these proteins, seventy-five accessions of T. turgidum ssp. turanicum, ssp. polonicum, ssp. carthlicum, previously selected by electrophoretic (lDE) and chromatographic (RP-HPLC) techniques, have been evaluated by two-dimensional electrophoresis (2DE) and polymerase chain reaction (PCR). Comparison of 2DE and PCR results have permitted the identification of B- and C-type LMW-GSs and of unknown putative C-type LMW-GSs in the tetraploid species and the designation of corresponding genes.