Enzymatic routes for the production of mono- and di-glucosylated derivatives of hydroxytyrosol

In this work, a new eco-friendly procedure for the synthesis of hydroxytyrosol and tyrosol alpha-glycosidic derivatives was proposed by using the marine alpha-glucosidase from Aplysia fasciata, and a commercial tyrosinase from mushroom for the bioconversion of tyrosol glycosidic derivatives into the corresponding hydroxytyrosol products. New hydroxytyrosol mono- and di-saccharide derivatives were synthesized at final concentrations of 9.35 and 10.8 g/l of reaction, respectively, and their antioxidant activity was evaluated by DPPH test. The best antioxidant agent resulted the (3,4-dihydroxyphenyl) ethyl-alpha-D-glucopyranoside: it showed a radical scavenging activity similar to that of the hydroxytyrosol, together with an increased hydrosolubility. This molecule could be a good response to many food industry demands, always in search of cheap antioxidants with nutritional properties to improve the nutritional value and the quality of foods. (C) 2011 Elsevier Ltd. All rights reserved.