Functional binding surface of a beta-hairpin VEGF receptor targeting peptide determined by nmr spectroscopy in living cells

In this study, the functional interaction of HPLW peptide with VEGFR2 (Vascular Endothelial Growth Factor Receptor 2) was determined by using fast (15) N-edited NMR spectroscopic experiments. To this aim, (15) N uniformly labelled HPLW has been added to Porcine Aortic Endothelial Cells. The acquisition of isotope-edited NMR spectroscopic experiments, including (15) N relaxation measurements, allowed a precise characterization of the in-cell HPLW epitope recognized by VEGFR2.