Reactive Deep Eutectic Solvents (RDESs): a new tool for Phospholipase D- catalyzed preparation of polar head modified phospholipids

In recent years, the development of new biocatalytic strategies aiming to achieve sustainable development goals is a highly investigated topic. To this end, Deep Eutectic Solvents (DESs) represent a valid greener alternative to common organic solvents in the preparation of polar head modified phospholipids (PLs) with phospholipase D (PLD)-catalyzed biotransformations. In this work, natural phosphatidylcholine (PC) has been submitted to PLD-catalyzed transphosphatidylation using new reaction media composed by tailored mixtures of DESs and buffer. These new solvents were able to solubilize PC as well as acting as nucleophilic reactants, thus avoiding the use of other toxic organic solvents usually necessary for these bioconversions.