Phospholipids hydrolysis in organic solvents catalysed by immobilised phospholipase C

Phospholipase C from Bacillus cereus has been immobilised on XAD7, Sepabeads FP-DA, Eupergit C, Celite 547, Silica gel 60 by covalent attachment or by adsorption. Preparates obtained with the two different immobilisation techniques show good hydrolytic activity in water-saturated organic solvents with phosphatidylcholine (PC) as a substrate. The chiral 1,2-diacyl glycerol can easily be obtained from the organic phase. The catalysts obtained by covalent attachment have been prepared with a higher specific activity and are suitable for repeated uses, while the ones prepared by adsorption with lower specific activity can only be used once. The initial rates of hydrolysis of PC solutions in different organic solvents and water content are compared.