Resolution by recombination: breaking up Solanum pennellii introgressions

Quantitative trait locus (QTL) genetics retains an important role in the study of biological and agronomic processes; however, its genetic resolution is often comparatively low. Community-based strategies are thus required to address this issue. Here we detail such a strategy wherein the widely used Solanum pennellii introgression lines (ILs) in the genetic background of the cultivated tomato (Solanum lycopersicum) are broken up into molecular marker-defined sublines as a community resource for map-based cloning.