Holographic imaging for tracking and phase retrieval in acoustophoresis platforms

Acoustophoresis devices are proposed as tools for manipulation and diagnostic in microfluidic environments. We demonstrate that their diffusion can be supported and enhanced by Digital Holography. Indeed, this technique covers all the current imaging needs and can stimulate the development of novel applications thanks to its unique features. The numerical refocusing is exploited to control the manipulation during acoustic focusing and acoustic-driven aggregation and to retrieve the 3D trajectories of tracer beads for the ultrasound field calibration. Besides tracking, DH displays its full potential when USs are used to directly manipulate or deform cells. In this case, numerical processing provides information on the sample movement and morphology, with potential applications in the field of diagnostic.