Publication Date:
2013
abstract:
We describe here the conjugation of polymethylmethacrylate nanoparticles to particular oligonucleotide switches, termed
molecular beacons (MBs), as potential intracellular nanosensors. Survivin mRNA targeting MBs have been used with
Atto647N and Blackberry 650 as fluorophore/quencher pair. The nanosensors have been characterized in vitro by
investigating the analytical performances of the chosen molecular beacon and its functionalities after conjugation to the
nanoparticles.
Iris type:
04.01 Contributo in Atti di convegno
Keywords:
Molecular bea; PMMA nanoparticles; intracellular sensing; mRNA; survivin
List of contributors:
Tuci, Giulia; Guerrini, Andrea; Giambastiani, Giuliano; Sotgiu, Giovanna; Varchi, Greta; Giannetti, Ambra; Trono, Cosimo; Tombelli, Sara; Ballestri, Marco; Baldini, Francesco
Book title:
Clinical and Biomedical Spectroscopy and Imaging III
Published in: