Oligonucleotide switches and nanomaterials for intracellular mRNA sensing
Contributo in Atti di convegno
Data di Pubblicazione:
2013
Abstract:
We describe here the conjugation of polymethylmethacrylate nanoparticles to particular oligonucleotide switches, termed
molecular beacons (MBs), as potential intracellular nanosensors. Survivin mRNA targeting MBs have been used with
Atto647N and Blackberry 650 as fluorophore/quencher pair. The nanosensors have been characterized in vitro by
investigating the analytical performances of the chosen molecular beacon and its functionalities after conjugation to the
nanoparticles.
Tipologia CRIS:
04.01 Contributo in Atti di convegno
Keywords:
Molecular bea; PMMA nanoparticles; intracellular sensing; mRNA; survivin
Elenco autori:
Tuci, Giulia; Guerrini, Andrea; Giambastiani, Giuliano; Sotgiu, Giovanna; Varchi, Greta; Giannetti, Ambra; Trono, Cosimo; Tombelli, Sara; Ballestri, Marco; Baldini, Francesco
Link alla scheda completa:
Titolo del libro:
Clinical and Biomedical Spectroscopy and Imaging III
Pubblicato in: