Reliability of detection of Citrus tristeza virus by an immunochromatographic lateral flow assay in comparison with ELISA

Citrus tristeza virus (CTV) is one of the most dangerous pathogens for Citrus spp.. Early diagnosis is essential for containment of the disease and many diagnostic techniques have been developed. But these need skilled personnel and laboratory equipment, and it takes some days for results to be obtained. We applied the easy-to-use technology known as Lateral Flow (LF) to detect CTV in orchard trees within few minutes of samples being taken. We compared LF results from leaf and fruit samples with those obtained by using ELISA and found a good correlation between these two techniques