Novel members of the family Micromonosporaceae, Rugosimonospora acidiphila gen. nov., sp. nov. and Rugosimonospora africana sp. nov.

Two novel Gram-positive-staining, acidophilic strains were isolated from soil samples. Both show typical features of filamentous actinomycetes. On the basis of 16S rRNA gene sequence analysis, the strains are members of the family Micromonosporaceae. The two strains contain hydroxydiaminopimelic acid, glycine, alanine and glutamic acid in the peptidoglycan. Fatty acid profiles clearly differentiate the two strains: cyclohexyl C17 : 0, i-C16 : 0 and ai-C17 : 0 are predominant in Delta1T, while the major components for Delta3T are ai-C17 : 0 and i-C16 : 0. The two strains also differ in their major menaquinones, MK-9(H8, H4, H6) for Delta1T and MK-9(H8, H6) for Delta3T, and in phospholipid patterns; Delta1T displays phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, methyl phosphatidylethanolamine and an unknown aminophospholipid, while Delta3T also contains minor amounts of several unknown phospholipids in addition to these phospholipids. The whole-cell sugars of both strains are galactose, arabinose and xylose. The G+C content of the DNA is 72.7 mol% for Delta1T and 71.9 mol% for Delta3T. On the basis of chemotaxonomic, physiological and phylogenetic data, we propose Rugosimonospora gen. nov. to accommodate the two strains, with the description of Rugosimonospora acidiphila gen. nov., sp. nov. (the type species; type strain Delta1T =DSM 45227T =NBRC 104874T) and Rugosimonospora africana sp. nov. (type strain Delta3T =DSM 45228T =NBRC 104875T).