Effective antibody anchoring on gold plate by ultra-short UV pulses

One of the main issues in biosensor research concerns the control of both the amount and the orientation of the bioreceptors. The so-called photonic induced immobilization can be very effective in anchoring antibodies with the variable part preferentially exposed, thus increasing the sensitivity of a Quartz Crystal Microbalance (QCM) immunosensor. In the present paper such a technique is applied to the detection of parathion, a relatively light analyte, demonstrating that the QCM is able to provide a detectable signal only if the photonic induced immobilization is applied, being completely insensitive to parathion when the bioreceptors are left to adhere randomly. © 2014 Springer Science+Business Media.