A multipurpose hybrid conventional/scanning near-field optical microscope for applications in materials science and biology

A hybrid conventional/scanning near-field optical microscope is presented. The instrument is obtained coupling an Olympus IX-70 inverted optical microscope with a SNOM head, to combine the versatility and ease of use of the conventional microscope with the high-resolution and three-dimensional reconstruction achieved by the SNOM. The head can be run in shear or tapping mode and is optimized to characterize soft, biological samples including living cells in physiological environment by including the SNOM in a cylindrical chamber that insulates it from external noise, while maintaining a controlled temperature and atmosphere.