Structure of low-order hemimorphite produced in a Zn-rich environment by cyanobacterium Leptolingbya frigida

Microbes play a fundamental role in the precipitation of silicate biominerals, thereby affecting the Si geochemical cycle. The fine mechanisms ruling biomineralization are not yet fully understood, and their microscopic structures can offer deep insight into their processes of formation, reactivity and stability. In this study, a Zn silicate biomineral, extracellularly produced by cyanobacterium Leptolingbya frigida, was investigated combining nuclear magnetic resonance (NMR), Zn K-edge X-ray absorption spectroscopy (XAS) and other complementary techniques. Si-29 magic angle spinning and Si-29/H-1 cross polarization magic angle spinning analysis, Fourier transform infrared spectroscopy (FTIR) and XAS analysis revealed a poorly crystalline phase closely resembling hemimorphite [Zn4Si2O7(OH)(2)center dot H2O]. Zn K-edge extended X-ray absorption fine structure (EXAFS) provided further structural details, revealing that the Zn-O-Si interatomic distances were 7-8% shorter than the abiotic mineral. C-13 NMR spectra analysis was conducted to investigate the composition of the Zn silicate biomineral organic matrix, and results revealed that C atoms occurred in several functional groups such as carbonyl carbons, C rings, O-aliphatic chains, N-aliphatic chains, and aliphatic chains.