UVB-radiation-induced apoptosis in Jurkat cells: a coordinated fourier transform infrared spectroscopy -flow cytometry study

we studied the induction of apoptosis in Jurkat cells try UVB radiation {wavelength 29s_!2t nm) at a dose of 310 mJi cm?. We combined Fotrier transform infrared (FTIR) spectroscopy with fow cytometry to determine whether the com' binatisn of both techniques could provide new and imprnved informaticn about celi modifications. To do this, n'e looked for correspondences and correlations between spectroscopy snd flory cytometry data and found three highly probable spectrcscopic markers of apoptosis' The behavior of the wave number strif't of troth the Amide I p-sheet componert and the area of the 1083 cm I band reprcduced, with a high correlation, the trehavinr of tke early apoptotic cell population, while the trehavior of the Amide I area shorved a high correlatian with the earty plus late apoptctic cell population'