Photofermentative Poly-3-Hydroxybutyrate Production by Rhodopseudomonas sp S16-VOGS3 in a Novel Outdoor 70-L Photobioreactor

In the present study, the performance of a 70 L photobioreactor, operating outdoors, was investigated using a purple bacterial strain as Rhodopseudomonas sp. S16-VOGS3 for producing poly-3-hydroxybutyrate (PHB). The novel photobioreactor was equipped with 5 rows L-shaped; the bottom of every row was placed in a stainless-steel tank containing water with controlled temperature. The photofermentation trials were carried out under fed-batch mode and under a semi-continuous regimen using lactic acid as the carbon source. The effect of the irradiance and the carbon/nitrogen ratio on the PHB accumulation was investigated, in order to evaluate the optimal bacterial growth. The results showed the feasibility of the prototype photobioreactor for the production of PHB by Rhodopseudomonas sp. S16-VOGS3 under the natural light/dark cycle. During the fed-batch growth (144 h long), the cumulative PHB increased quickly reaching a maximum value of 377 mg/L and decreased to 255 mg/L during the semi-continuous regimen (336 h long).