Direct PCR Analysis of Biological Samples in Disposable Plastic Microreactors for Biochip Applications

In this work we show the development and assessment of disposable, biocompatible, fully plastic microreactors, which are demonstrated to be highly efficient for performing on-chip direct PCR analyses of very diverse crude biological samples, such as human cells, whole blood, buccal scrape and hair bulbs, without requiring any purification steps before PCR analyses.