BLUE LED light effects in an in vivo murine model of ulcer

Aim: To investigate the molecular effects of 30s treatment with a Blue LED device on a murine model ulcer. Method: 63 CD1 mice were anesthetized and randomized in three groups: GI, GII, GIII. In GI and GII groups one full-thickness wound on the shaved back was performed with a 4mm large punch, while in GIII group two wounds were induced. The animals of the GI group and only one lesion of GIII group underwent a 30s treatment with a Blue LED light, while the GII group were left to naturally recover. The animals were sacrificed after 1, 3, 6, 9, 24 hours and 7 and 14 days after the treatment and skin biopsies from the back were obtained. During all the treatments, the superficial skin temperature was monitored with a thermal camera. A customized ELISA kit enabled to study EGF, bFGF, VEGF, TNF-?, MMP-2 and PRO-MMP-9 at different postoperative time points; moreover, a histological analysis with hematoxylin-eosin method was performed. Results / Discussion: The expression of TNF-alpha, bFGF and Pro-MMP-9 evidenced a modulation due to the light treatment. The GIII group shows differences in the healing process between the treated and untreated wound. Conclusion: These experimental results are supporting our previous studies: the Blue LED light treatment is a modulator of the wound healing process. Its application can be extremely useful in the treatment of ulcers and chronic wounds.