Nanostructured gold for immobilization of thioaniline functionalized glucose oxidase and Au nanoparticles by electropolymerization

Sensors are devices composed of an active sensing material with a signal transducer. Electrochemical sensors have more advantages over the others because the electrodes can sense the analyte that is present in the host without doing any damage to the host system. The immobilization of a protein on a metallic transducer can be a crucial step in the development of bionanodevices that find applications in the field of biomaterials, biocoatings, biofuel cells, etc. In the present study, we show the immobilization by electropolymerization of thioaniline functionalized glucose oxidase and Au nanoparticles on nanostructured gold films prepared by electrodeposition and compared to sputtered gold. This enzyme is employed in the preparation of biosensors of biomedical interest. The goodness of the Au film for enzyme binding is evaluated by comparison of the enzyme activity and of the interval of linearity for the determination of glucose concentration. © The Electrochemical Society.