In vivo assessment of skin impedance recovery after low-voltage pulses electroporation

The aim of this work is to measure the skin impedance recovery after electroporation by using low-voltage pulse protocol on human subject. Before and after electroporation, based on square wave pulses of 20-50 V, 5 ?s, for 15-20 minutes, Electrical Impedance Spectroscopy (EIS) measurements has been performed, in the frequency range 0.5-10 kHz. Preliminary experimental results show a significant decrease in skin impedance amplitude after treatment. This result turns out to be useful in a measurement procedure aimed at determining the amount of drug actually transferred in electroporation-based therapy.