The energy profiling of short interfering RNAs is highly predictive of their activity

The technology of knockdown of gene expression by synthetic short interfering RNAs (siRNAs) is currently diffused because of its relative simplicity and reliability. Despite the claims that active siRNAs can be easily identified, how to select active siRNAs with high efficiency it is not fully defined. Here, we report that energy profiling can be used in the guidelines for siRNA design.