Publication Date:
2022
abstract:
The production of recombinant proteins in bacteria made possible to obtain large quantities of proteins essential for basic and applied research. Escherichia coli remains one of the organisms of choice for recombinant proteins because of its ability to grow at high density and availability of a vast catalog of cloning vectors and mutant host strains. Here, we describe the protocols for the expression of cold-adapted (hemo)globins in Escherichia coli.
Iris type:
02.01 Contributo in volume (Capitolo o Saggio)
Keywords:
Bacterial hemoprotein; Cold adaptation; Escherichia coli; Expression plasmid; Recombinant-protein expression; UV-visible absorption spectrum.
List of contributors:
Verde, Vincenza; Giordano, Daniela
Book title:
Marine Genomics
Published in: