AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody

Author summary To complete cell division, the bridge that links the two daughter cells needs to be cleaved. This step is carried out by a machinery named "endosomal sorting complex required for transport" (ESCRT). The dissection of this machinery is important in basic biology and for investigating diseases in which cell division is altered. AKTIP, a factor discovered to be needed for chromosome integrity, shares similarities with a component of the ESCRT machinery named TSG101. Here we present evidence that AKTIP is part of the ESCRT machinery, as TSG101. More specifically, we show that AKTIP physically interacts with members of the ESCRT machinery and forms a characteristic circular structure at the center of the bridge linking the daughter cells. We also show that the reduction of AKTIP levels causes defects in the assembly of the ESCRT machinery and in cell division. In future work, it will be interesting to investigate the association of AKTIP with cancer, because in tumorigenesis cell division is altered and since an implication in cancer has been described for TSG101 and other ESCRT factors.