Publication Date:
2018
abstract:
The endoplasmic reticulum takes care of the folding, assembly and quality control of thousands of proteins destined to the different compartments of the endomembrane system, or to be secreted in the apoplast. Here we describe how these early events in the life of all these proteins can be followed biochemically by using velocity or isopycnic ultracentrifugation, metabolic labelling with radioactive amino acids and immunoprecipitation in various conditions.
Iris type:
02.01 Contributo in volume (Capitolo o Saggio)
Keywords:
Protein synthesis; Endoplasmi; chaperones; protein folding; protein oligomerization; protein-protein interaction; pulse-chase; immunoprecipitation; ultracentrifugation
List of contributors:
Pedrazzini, Emanuela; Vitale, Alessandro
Book title:
The Plant Endoplasmic Reticulum. Methods and Protocols
Published in: