Cigarette smoke increases TLR4 expression and modifies LPS mediated responses in airway epithelial cells.
Conference Poster
Publication Date:
2007
abstract:
Airwayepitheliumisemergingasaregulatorofinnateimmuneresponsestoa
varietyofinsultsincludingcigarettesmoke.Themaingoalofthisstudywas
to exploretheeffectsofcigarettesmokeextracts(CSE)inthetolllikereceptor
(TLR) expressionandactivationinahumanbronchialepithelialcellline(16-
HBE). CSEincreasedtheexpressionofTLR4andtheLPSbinding,theNFkB
activation,thereleaseofIL-8andthechemotacticactivitytowardneutrophilsbut
did notinducetheTLR2expressionandtheERK1/2activation.LPSincreasedthe
expressionofTLR4andinducedbothNFkBandErk1/2activation.Thecombined
exposureof16-HBEtoCSEandLPS,furtherincreasedIL-8releaseandthe
chemotactic activitytowardneutrophils.Theseadditiveeffectswereassociated
to ERKactivationratherthantoNFkBactivation.Furthermore,CSEdecreased
the constitutiveIP-10releaseandcounteractedtheeffectofLPSininducing
IP-10 release.Inconclusion,cigarettesmokecontributestotheaccumulationof
neutrophils withintheairwaysofsmokersbyincreasingtheexpressionofTLR4
and bypreferentiallyorientatingTLR4activationtowardactivationofERKand
IL-8 production.
SupportedbyGlaxoSmithKline
Iris type:
04.03 Poster in Atti di convegno
List of contributors:
Melis, MARIO RAPHAEL; Pace, Elisabetta; Gjomarkaj, Mark; Bonsignore, Giovanni; Siena, Liboria; Bonsignore, MARIA ROSARIA
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