Screening of a BAC Library of Citrus Sinensis by means of Overgo Probes for the Identification of Clones Containing Gene Sequences with Relevant Roles in Fruit Quality.

Compared with the most commonly used gene-specific hybridization probes (subcloned DNA fragments, PCR amplimer products or DNA oligonucleotides), the overgo hybridization method (Ross et al., 1999) has several advantages (better hybridization kinetics, higher specificity labeling, low background of hybridization, low rate of false positives, high throughput, and ease of handling). The foundation of these advantages is the short sequence that is needed for an overgo probe, which increases the likelihood of finding suitable single copy regions from EST sequences or unigenes.