Biological Effects of Transforming Growth Factor Beta in Human Cholangiocytes

TGF-beta is a cytokine implicated in multiple cellular responses, including cell cycle regu-lation, fibrogenesis, angiogenesis and immune modulation. In response to pro-inflammatory and chemotactic cytokines and growth factors, cholangiocytes prime biliary damage, characteristic of cholangiopathies and pathologies that affect biliary tree. The effects and signaling related to TGF-beta in cholangiocyte remains poorly investigated. In this study, the cellular response of human cholan-giocytes to TGF-beta was examined. Wound-healing assay, proliferation assay and cell cycle analyses were used to monitor the changes in cholangiocyte behavior following 24 and 48 h of TGF-beta stimula-tion. Moreover, proteomic approach was used to identify proteins modulated by TGF-beta treatment. Our study highlighted a reduction in cholangiocyte proliferation and a cell cycle arrest in G0/G1 phase following TGF-beta treatment. Moreover, proteomic analysis allowed the identification of four downregulated proteins (CaM kinase II subunit delta, caveolin-1, NipSnap1 and calumin) involved in Ca2+ homeostasis. Accordingly, Gene Ontology analysis highlighted that the plasma membrane and endoplasmic reticulum are the cellular compartments most affected by TGF-beta. These results suggested that the effects of TGF-beta in human cholangiocytes could be related to an imbalance of intracellular calcium homeostasis. In addition, for the first time, we correlated calumin and NipSnap1 to TGF-beta signaling.