Isolation and in vitro translation of mRNA from inflorescences of Parietaria judaica

The aqueous extract of inflorescences of Parietaria judaica contains an allergen homologous to the major pollen allergen Par o I (14 kD), as shown by radio-allergosorbent test (RAST) inhibition and immuno-blot analysis. Poly(A)⁺ RNA was obtained from inflorescences and was shown to be able to code in vitro for a protein homologous to Paro I with respect to sodium dodecylsulphate polyacrylamidegel electrophoretic mobility and to antigenic specificity as defined by the binding, in affinity chromatography, to solid-phase IgG of rabbit anti- Paro /antisera, and in RAST inhibition, to IgE antibodies of human reaginic serum pool. © 1990 S. Karger AG, Basel.